论文部分内容阅读
目的观察柴胡桂枝汤对实验性大鼠肝纤维化Ⅰ型纤溶酶原激活抑制物(PAI-1)及Ⅲ型前胶原氨基端肽(PⅢNP)表达的影响。方法采用CCl4复合法制备肝纤维化大鼠模型,给予相应药物灌胃治疗。8周末处死大鼠,用免疫组化法及酶联免疫法检测大鼠肝脏组织中PAI-1的表达情况和血清中PⅢNP的含量。结果柴胡桂枝汤治疗组与模型组相比,PAI-1的表达及PⅢNP的含量显著降低,PAI-1的表达及PⅢNP含量下降呈明显正相关。结论柴胡桂枝汤能降低肝脏PAI-1的表达和血清中PⅢNP的含量,具有抗纤维化作用。
Objective To observe the effects of Chaihu Guizhi Decoction on the expression of type I plasminogen activator inhibitor (PAI-1) and type III procollagen amino-telopeptide (PIIINP) in experimental rat liver fibrosis. Methods The rat model of hepatic fibrosis was prepared by CCl4 complex method, and the corresponding drugs were given by gavage. The rats were sacrificed at the end of the 8th week. The expression of PAI-1 in the liver tissue and the content of PIIINP in serum were detected by immunohistochemistry and enzyme-linked immunosorbent assay. Results Compared with the model group, the expression of PAI-1 and PIIINP in the treatment group of Chaihu Guizhi Decoction were significantly decreased, and the expression of PAI-1 and the decrease of PIIINP content were positively correlated. Conclusion Chaihu Guizhi Decoction can reduce the expression of PAI-1 in the liver and the content of PIIINP in serum, which has anti-fibrotic effect.