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目的研究氧化魔芋葡甘露聚糖(oxidized konjac glucomannan,OKGM)对小鼠免疫功能的影响,探讨其对小鼠的免疫调节作用。方法 200只雌性昆明种小鼠随机分成正常对照组(灌胃蒸馏水)和阳性组(灌胃具有免疫增强功能的保健食品氨基酸口服液)及OKGM低、中、高剂量组(0.075、0.75、2.25g/kg bw)共5个试验组。每日1次进行灌胃,其间进行小鼠生长情况观察,连续30d后进行脏器/体重比值测定、NK细胞活性、碳廓清实验及腹腔巨噬细胞吞噬鸡红细胞(cock red blood cells,CRBC)的实验、抗体生成细胞检测和血清溶血素实验。结果对小鼠的体重增长和胸腺及脾脏指数无影响。小鼠NK细胞活性:在低、中、高剂量组、阳性对照组与正常对照组间比较有显著的差异(P<0.05),且具有剂量差异性。小鼠碳廓清实验:在低、中、高剂量组、阳性组与正常对照组间比,吞噬指数均差异非常显著(P<0.01),且呈现明显的剂量效应。腹腔巨噬细胞吞噬鸡红细胞实验:吞噬率和吞噬指数在中、高剂量组、阳性对照组与正常对照组间比均差异非常显著(P<0.01),而低剂量组与正常对照组比较无显著差异(P>0.05)。抗体生成细胞数:在低、中、高剂量组和阳性对照组与阴性对照组间比均差异非常显著(P<0.01),且产生的空斑数呈剂量效应,但各剂量组的抗体生成细胞数均不及阳性对照组高。小鼠血清溶血数(血凝法)实验:与阴性对照组比,低剂量组和阳性组均差异显著(P<0.05),而中、高剂量组则差异非常显著(P<0.01),且呈剂量效应。说明小鼠NK细胞活性、小鼠单核-巨噬细胞实验、小鼠体液免疫功能实验结果均呈阳性。结论OKGM能增强小鼠免疫功能。
Objective To investigate the effect of oxidized konjac glucomannan (OxGM) on immune function in mice and its immunomodulatory effect on mice. Methods 200 female Kunming mice were randomly divided into normal control group (intragastric distilled water) and positive group (oral administration of amino acid oral solution of health food with immune function) and OKGM low, medium and high dose groups (0.075, 0.75, 2.25 g / kg bw) a total of five experimental groups. The mice were gavaged once a day for observation of the growth of the mice. The organ / body weight ratio was measured 30 days after the continuous administration. NK cell activity, carbon clearance test and peritoneal macrophage phagocytosis of chicken red blood cells (CRBC) Of the experiment, antibody-producing cells and serum hemolysin test. Results have no effect on body weight gain and thymus and spleen index in mice. The NK cell activity of mice: There were significant differences (P <0.05) between the positive control group and the low, medium and high dose groups, and with dose difference. The carbon clearance test in mice: Compared with the normal control group, the phagocytic index was significantly different (P <0.01) in the low, medium and high dose groups, and showed a significant dose effect. Peritoneal macrophage phagocytosis of chicken erythrocytes: The phagocytic rate and phagocytic index in the medium and high dose groups, positive control group and the normal control group were significantly different (P <0.01), while the low dose group compared with the normal control group Significant difference (P> 0.05). The number of antibody-producing cells: There was a significant difference (P <0.01) between the low, medium and high dose groups and the positive control group and the negative control group, and the number of plaques produced was dose-effect. However, the antibody production Cells are not as high as the positive control group. Compared with the negative control group, there was significant difference between the low dose group and the positive group (P <0.05), while the middle and high dose groups showed significant difference (P <0.01) Dose effect. The results showed that the activity of NK cells in mice, the results of mouse monocyte-macrophage and the humoral immunity of mice showed positive results. Conclusion OKGM can enhance the immune function in mice.