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以西瓜(Citrullus vulgarris)郑抗1号为接穗,日本南瓜(C.moschata)、黑籽南瓜(C.ficifolia)、葫芦(Lagenaria siceraia)为砧木,研究了嫁接苗和自根苗在(7.5±0.5)℃低温胁迫下叶片相关耐寒性指标的变化。结果表明:低温处理后嫁接苗丙二醛(MDA)含量、电解质渗透率显著低于自根苗;叶绿素、脯氨酸(Pro)、可溶性糖含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著高于自根苗。与处理前相比,12d时日本南瓜砧、黑籽南瓜砧和葫芦砧嫁接苗叶绿素含量分别降低了30.48%、28.48%和52.69%;葫芦砧嫁接苗MDA含量6d时较处理前增加了313.16%;3～12d日本南瓜砧和黑籽南瓜砧嫁接苗电解质渗透率显著低于葫芦砧嫁接苗;低温处理期间,日本南瓜砧嫁接苗可溶性糖含量分别比黑籽南瓜砧和葫芦砧嫁接苗高出24.71%和31.17%,SOD、POD、CAT酶活性高于另外2个组合。综合各项指标显示:3种砧木均能提高接穗对低温的忍耐能力,其顺序为日本南瓜砧>黑籽南瓜砧>葫芦砧。
The results showed that the grafted seedlings and self-rooted seedlings were harvested at the level of (7.5 ± 0.5), with the roots of Citrullus vulgarris Zhengkang 1 as scion, C.moschata, C.ficifolia and Lagenaria siceraia as rootstocks, Changes of leaf related cold tolerance index under low temperature stress. The results showed that the contents of malondialdehyde (MDA) and electrolyte in grafted seedlings after low temperature treatment were significantly lower than those of self-rooted seedlings. The content of chlorophyll, proline, soluble sugar and superoxide dismutase (SOD) Enzyme (POD) and catalase (CAT) activities were significantly higher than those of self-rooted shoots. Compared with those before treatment, the chlorophyll content of grafted Japanese pumpkin, black seed pumpkin and gourd anvil decreased by 30.48%, 28.48% and 52.69% respectively at 12 d. MDA content of grafted seedlings increased 313.16% ; 3 ~ 12d Japan pumpkin anvil and black seedling pumpkin grafted seedling electrolyte permeability was significantly lower than the gourd rootstock grafted seedlings; low temperature treatment, the Japanese pumpkin anvil grafted seedlings soluble sugar content were higher than the black-seed pumpkin anvil and gourd grafted grafted seedlings 24.71% and 31.17% respectively. SOD, POD and CAT activities were higher than the other two combinations. Comprehensive indicators show that: 3 kinds of rootstocks can improve scion tolerance to low temperature, the order of Japanese pumpkin anvil> black seed pumpkin anvil> gourd anvil.