目的 :观察重组人肿瘤坏死因子 α(rhTNF α)对建系细胞 (T T)作用效应与机制。方法 :反转录PCR方法克隆人TNFR1基因 ,并将其稳定转染入舌癌细胞中 ,建系并鉴定 ;通过MTT法检测、DNA降解片段琼脂糖凝胶电泳及电镜观察检测rhTNF α对T T细胞的作用效果与机制。结果 :成功构建含TNFR1基因的真核表达质粒 pcDNA3 TNFR1,并建立表达TNFR1蛋白活性的舌癌细胞系T T ;较之亲本Tca 8113细胞及转染空载体的T pc细胞 ,rhTNF α对T T细胞具有更强的细胞毒作用 ,且通过介导细胞凋亡的方式发挥其作用。结论 :重组人肿瘤坏死因子 α联合肿瘤坏死因子受体 1基因转染可有效地杀伤舌癌细胞 ,为舌癌基因治疗提供理论依据。 Objective: To observe the effect and mechanism of recombinant human tumor necrosis factor α (rhTNF α) on the T cells. METHODS: The human TNFR1 gene was cloned by RT-PCR and stably transfected into tongue cancer cells. The MTT method was used to detect the expression of rhTNFα by TTT. The DNA fragment was extracted by agarose gel electrophoresis and electron microscopy. The effect and mechanism of cells. Results: The eukaryotic expression plasmid pcDNA3 TNFR1 containing TNFR1 gene was successfully constructed and the tongue cancer cell line TT expressing the activity of TNFR1 protein was established. Compared with Tca 8113 cells transfected with empty vector and T pc cells transfected with empty vector, rhTNF α had Stronger cytotoxicity, and exert its effect by way of mediating apoptosis. Conclusion: Transfection of recombinant human tumor necrosis factor-α and tumor necrosis factor-1 receptor gene can effectively kill tongue cancer cells and provide a theoretical basis for gene therapy of tongue cancer.