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A simple,precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets.According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II,a small glass-method was adopted at the rotational speed of 100 r/min using 100 mL phosphate buffer(pH 6.8) as release medium.The release amount was determined by HPLC with a C 18 column(250 mm 4.6 mm,5μm) using the mobile phase consisting of methanol 0.4% carboxylic acid(65:35) at a flow rate of 1 mL/min and UV detection at 242 nm.The current method demonstrates good linearity over the range 4.052-405.2 μg/mL(r2=0.9999) with an average recovery of 105.5%(RSD =1.25%).The accumulative release of alliin/alliinase double-layer tablets had good homogeneity for within-and betweenbatches.The method established is simple,accurate and repeatable for the determination of allicin release from alliin/alliinase double-layer tablets.
A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin / alliinase double-layer tablets. According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100 r / min using 100 mL phosphate buffer (pH 6.8) as release medium. The release amount was determined by HPLC with a C 18 column (250 mm 4.6 mm, 5 μm) using the mobile phase consisting of methanol 0.4% carboxylic acid ( 65:35) at a flow rate of 1 mL / min and UV detection at 242 nm. The current method demonstrates good linearity over the range 4.052-405.2 μg / mL (r2 = 0.9999) with an average recovery of 105.5% (RSD = 1.25%). The accumulative release of alliin / alliinase double-layer tablets had good homogeneity for within-and betweenbatches. The method established is simple, accurate and repeatable for the determination of allicin release from alliin / alliinase double-layer tablets.