论文部分内容阅读
在构建成人睾丸cDNA文库的基础上,用兔抗人精子抗体自文库中筛选出了8株阳性克隆,并对其中的HSG2表达产物进行理化特性测定和抗HSG2表达产物抗体的生物学活性测定。SDS-PAGE电泳显示溶原蛋白的分子量约为155KDa。抗HSG2表达产物抗体能明显的抑制精子的运动能力,对精子凝聚、顶体蛋白酶和精子表面的ConA受体无影响。提示HSG2表达产物可能成为精子疫苗的候选成份。
Based on the construction of adult testicular cDNA library, 8 positive clones were screened from the library by using rabbit anti-human sperm antibody. The physical and chemical properties of HSG2 expression product and the biological activity of anti-HSG2 antibody were determined. SDS-PAGE electrophoresis showed that the molecular weight of the protein is about 155KDa. Anti-HSG2 expression product antibody can significantly inhibit sperm motility, sperm aggregation, acrosin protease and sperm surface ConA receptor no effect. Tip HSG2 expression product may become a candidate component of sperm vaccine.