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目的探析佛山市早期妇女叶酸代谢障碍相关基因位点分布与胎儿生长障碍(FGR)的相关性研究。方法对2014年8月-2016年8月期间在佛山市妇幼保健院产前检查的孕妇(单胎)1500例,从确诊为FGR的病例中取100例FGR作为研究组,根据FGR组中孕妇的年龄、孕龄、户籍资料等。选取100例具有可比性的正常孕妇作为对照组,分别从其孕5~8周起监测同型半胱氨酸值及血清叶酸水平情况,对叶酸代谢障碍的妇女进行叶酸代谢障碍相关遗传基因位点多态性进行检测。结果 (1)FGR研究组与对照组相比,其中C677T、A1298C、A66G型中TT、CC、GG 3种位点基因型的血浆中Hcy水平均明显高于对照组,差异均有统计学意义(P<0.05)。总的突变T等位基因、突变C等位基因及G等位基因频率显著高于对照组,差异均有统计学意义(P<0.05)。(2)与C677T的CC型相比,CT型、TT型与CT+TT型会增加叶酸代谢障碍的发病概率,差异有统计学意义(P<0.05);与A1298C的AA型相比,AC型、CC型与AC+CC型会增加叶酸代谢障碍的发病概率,差异有统计学意义(P<0.05);与A66G的AA型相比,AG型、GG型与AG型+GG型会增加叶酸代谢障碍的发病概率,差异有统计学意义(P<0.05)。结论佛山地区孕早期妇女叶酸代谢障碍相关基因位点分布与FGR有关。
Objective To investigate the correlation between locus distribution of folic acid metabolism disorder and fetal growth disorder (FGR) in early women in Foshan City. Methods One hundred and fourteen pregnant women (singleton) in prenatal care at Foshan Maternal and Child Health Hospital from August 2014 to August 2016 were enrolled in this study. 100 FGR patients were enrolled in the study. The age, gestational age, household registration information. 100 matched normal pregnant women were selected as the control group. The homocysteine level and serum folate level were monitored from the 5th to the 8th week of gestation. The women with folic acid metabolism were evaluated for the association of folic acid metabolism-related genetic loci Polymorphism was tested. Results (1) Compared with the control group, the Hcy levels in plasma of the three genotypes of TT, CC and GG in C677T, A1298C and A66G groups were significantly higher in FGR group than in control group, the differences were statistically significant (P <0.05). The total mutation T allele, mutation C allele and G allele frequency were significantly higher than those in the control group, the difference was statistically significant (P <0.05). (2) Compared with CC type of C677T, CT type, TT type and CT + TT type increased the incidence of folic acid metabolism disorder, the difference was statistically significant (P <0.05). Compared with AA type of A1298C, AC Type, type CC and type AC + CC increased the incidence of folic acid metabolism disorders, the difference was statistically significant (P <0.05); compared with AA type A66G, AG type, GG type and AG type + GG type will increase The incidence of folic acid metabolism disorders, the difference was statistically significant (P <0.05). Conclusion The distribution of FAD related genes in pregnant women in early pregnancy in Foshan is related to FGR.