为了获得性状优良的毛木耳新品种,进行了杂交育种。实验以钩悬法收集毛木耳“丰2”子实体单孢子并培养获得其单核菌丝。采用单孢杂交(两点法)进行杂交培养,镜检“锁状联合”初选杂交子;通过与亲本的拮抗实验和酯酶同工酶电泳酶谱比对分析确认杂交子,并经出耳试验验证其结实性。实验结果显示,种内杂交的不同单核菌丝较易亲和并产生具结实能力的杂交子。 In order to obtain a good trait of the new fungus varieties, conducted a cross breeding. Experiments were hook-hanging method to collect hairy wood “Feng 2 ” fruiting bodies of single spore and cultured to obtain its mononuclear hyphae. Hybridization was carried out by single-spike hybridization (two-point method), microscopic examination of the “lock-like combination” primary hybrids; hybridization was confirmed by antagonistic experiments with esterase isoenzyme zymogram analysis and The ear test to verify its robustness. The experimental results show that different mononuclear hyphae hybridized within the species more pro-affinity and produce a robust hybrid ability.