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在酶的结构中,疏水部位的底物结合作用(Substrate binding)是酶的一个非常主要的功能.在酶的功能模拟中,引入类似于酶的微区对于理解酶的特殊催化能力具有重要意义.前文报道了不等性微区概念的提出并用 NMR 技术表征了聚乙撑亚胺(PEI)衍生物的疏水微区.本文利用甲基橙(MO)染料作探针进一步表征了 PEI 衍生物的疏水微区.甲基橙的最大吸收蜂(λ_(max))在低极性的介质中移向低波长:在水中λ_(max) 为465nm,在有机溶剂中为420—430nm.这一位移已被用于表征溶液中聚合物的微环境.因此,由部分季铵化的 PEI 提供的微环境的本质同样可以通过 PEI 衍生物对 MO 紫外吸收的影响来进行研究.
Substrate binding at the hydrophobic site is a very important function of the enzyme in the structure of the enzyme.Introducing enzyme-like domains into the functional modeling of enzymes is of great importance to understand the special catalytic ability of enzymes We previously reported the concept of unequal microdomains and used NMR techniques to characterize the hydrophobic domains of polyethylenimine (PEI) derivatives.In this paper, methyl orange (MO) dye was used as a probe to further characterize PEI derivatives (Λ max) of methyl orange shifts to lower wavelengths in low polarity media: λ max (max) 465 nm in water and 420-430 nm in organic solvents Displacement has been used to characterize the microenvironment of the polymer in solution Thus, the nature of the microenvironment provided by partially quaternized PEI can likewise be investigated by the effect of PEI derivatives on MO UV absorption.