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目的 :研究维生素C对胆固醇和甘油三酯测定的干扰及机理.方法 :用COD PAP法测定胆固醇 ,用GPO PAP法测定甘油三酯.结果 :对血脂浓度相同、维生素C浓度不同的系列标本进行测定时 ,所测胆固醇和甘油三酯浓度均随着维生素C浓度的增高而降低 ;当测定不含血脂的过氧化氢溶液时 ,胆固醇和甘油三酯的实测浓度均随着过氧化氢的稀释而降低 ;当过氧化氢浓度相同时 ,所测得胆固醇和甘油三酯浓度均随着维生素C浓度的增高而降低.结论 :维生素C可干扰COD PAP法对胆固醇的测定 ,干扰GPO PAP法对甘油三酯的测定 ,导致两种测定结果假性降低 ;维生素C可能通过使反应的中间产物过氧化氢和最终产物苯醌亚胺还原而干扰胆固醇和甘油三酯的测定.“,”Objective: To study the interference and mechanism of vitamin C on determination of cholesterol and triglycerides. Methods: Cholesterol concentrations were determined with COD PAP method and triglycerides concentrations were determined with GPO PAP method. Results: When a series of samples containing the same concentration of lipids but different concentrations of vitamin C were determined, the determined cholesterol and triglycerides concentrations decreased with the increase of vitamin C concentrations in the sample. When lipid free hydrogen peroxide solutions were determined, the determined cholesterol and triglyceride concentrations decreased with dilutions of hydrogen peroxide. When hydrogen peroxide concentration remained constant in solutions , the determined cholesterol and triglyceride concentrations decreased with the increase of vitamin C concentrations in the solutions . Conclusion: Vitamin C can interfere with the determination of cholesterol with COD PAP method, and the determination of triglycerides with GPO PAP method, resulting in false lower cholesterol and triglyceride concentrations. Vitamin C interferes with the determination of cholesterol and triglycerides possibly through the reduction of the intermittent product hydrogen peroxide and final product quinoneimine.