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目的研究KCI诱导皮层扩散性抑制(CSD)预处理对大鼠大脑中动脉阻塞后脑血流灌注和梗死体积的影响,验证CSD对脑缺血性损伤的保护作用。方法24只SD大鼠分为实验组和对照组,各12只,试验组用5 mol/L KCl大鼠大脑皮层诱发CSD,3d后插线法栓塞大脑中动脉制备大鼠局灶性脑缺血模型,利用激光散斑衬比光学成像技术监测缺血2h大脑血流动力学变化,并测定大脑梗死病灶体积。对照组用NaCI代替KCI,其余相同。比较血流动力学变化和大脑梗死灶体积的大小。结果经CSD预处理后3d再缺血时,实验组缺血病灶血液灌注水平高于对照组,实验组总梗死体积及皮层梗死体积均小于对照组,皮层下梗死体积与对照组差异无统计学意义(P＞0．05)。结论利用激光散斑衬比光学成像技术可以在体检测脑血流的变化,同时验证了CSD预处理可提高缺血病灶皮层的血液灌注水平,减少总梗死体积和皮层梗死体积,可以减轻脑缺血后的脑功能损害程度。
Objective To investigate the effect of CSD preconditioning on cerebral blood flow perfusion and infarct volume after middle cerebral artery occlusion in rats and to verify the protective effect of CSD on cerebral ischemic injury. Methods Twenty-four SD rats were divided into experimental group and control group, with 12 rats in each group. CSD was induced by 5 mol / L KCl in cerebral cortex in rats, and focal cerebral ischemia Blood model, the use of laser speckle contrast-imaging technique to monitor hemodynamic changes in cerebral infarction 2h, and determine the volume of cerebral infarction lesions. The control group instead of KCI NaCI, the rest of the same. Compare the hemodynamic changes with the size of cerebral infarction volume. Results After reperfusion for 3 days after CSD preconditioning, the level of blood perfusion in the experimental group was higher than that in the control group. The total infarct volume and cortical infarct volume in the experimental group were less than those in the control group. There was no significant difference in infarct volume between the experimental group and the control group Significance (P> 0.05). Conclusions Laser speckle contrast imaging can detect cerebral blood flow changes in vivo. It is also demonstrated that CSD preconditioning can increase the blood perfusion of ischemic cortex, reduce the total infarct volume and infarct volume, Blood damage to brain function.