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洪雅县自1965年开展血防工作以来,反复采用皮试、IHA、ELISA、粪检等方法先后调查100多万人次,一直未检出确诊的血吸虫病例,因此,四川省市血防专业机构把该县定为“无病有螺县”。1992年,我们在采用四川省寄研所EIA方法快速检测日本血吸虫循环抗原的基础上,结合病原学检查(直肠活组织镜检),首次在县境内确诊出慢性血吸虫病人。 1 材料与方法 1.1 循环抗原检测试剂及对照参考血清均系四川省医科院寄研所提供。 1.2 循环抗原测定检测对象为新庙乡介牌村3-60岁人群共851人。采受检者耳垂血约0.2ml于塑料管内,其一端加热密封,经1500-2000转/min离心3min,分离血清。在PVC复合载体的每凹孔内
Hongya County since 1965 to carry out blood work since the repeated use of skin test, IHA, ELISA, fecal examination and other methods have been investigated more than 100 million people, has not been detected in the diagnosis of schistosomiasis cases, therefore, County as “disease-free Ariake County.” In 1992, based on the rapid detection of circulating antigens of Schistosoma japonicum by EIA in Sichuan Provincial Institute of Education and Research, combined with etiological examination (rectal biopsies), we first diagnosed patients with chronic schistosomiasis in the county. 1 Materials and Methods 1.1 Cyclic antigen test reagents and reference serum are provided by the Sichuan Provincial Institute of Medical Research Institute. 1.2 Determination of circulating antigen test object for the new temple village 3-60 years old, a total of 851 people. Taken by the test ear lobe blood about 0.2ml in plastic tube, one end of the heat sealed by centrifugation 1500-2000 rev / min 3min, serum was separated. Within each cavity of the PVC composite carrier