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The microbial transformation of glycyrrhetinic acid(1) by Cunninghamella blakesleana CGMCC 3.970 led to the production of five new metabolites(2-6).The structures of the metabolites were determined by extensive spectroscopic(HR-ESIMS,1D and 2D NMR) data analyses.The involved reactions exhibited specific hydroxylations at C-24,C-7,and C-15,and oxidation at C-3.Moreover,compounds 2,5,and 6showed significant neural anti-inflammatory activity by inhibiting lipopolysaccharide-induced NO production in mouse microglia BV2 cells with IC_(50) values of 0.76,0.94,and 0.16μmol/L,respectively.
The microbial transformation of glycyrrhetinic acid (1) by Cunninghamella blakesleana CGMCC 3.970 led to the production of five new metabolites (2-6). The structures of the metabolites were determined by extensive spectroscopic (HR-ESIMS, 1D and 2D NMR) data analyzes . These involved reactions suggest specific hydroxylations at C-24, C-7, and C-15, and oxidation at C-3. Moreover, compounds 2,5, and 6showed significant neural anti-inflammatory activity by inhibiting lipopolysaccharide- induced NO production in mouse microglia BV2 cells with IC 50 values of 0.76, 0.94, and 0.16 μmol / L, respectively.