论文部分内容阅读
目的为了解人芽囊原虫对外界条件的适应性,观察培养条件改变对其形态和繁殖方式的影响。方法从腹泻患者粪便中检查并分离培养获得人芽囊原虫,分别用locke氏-鸡蛋双相培养基(LES)和改良1640培养基(RPMI-1640)培养,改变培养条件,观察人芽囊原虫形态和繁殖方式。结果在两种培养基中均可见到人芽囊原虫的四种主要形态:空泡型、颗粒型、阿米巴型和包囊型,其繁殖方式以二分裂为主,可见少量出芽生殖。当从LES培养基突然变为RPMI-1640培养基时,人芽囊原虫出现短时间(48～72h)内生长停滞,随后出现多种形式的繁殖方式,可见较多的二分裂、出芽生殖、三分裂、多分裂、裂体生殖,持续24h后恢复为二分裂为主。结论培养条件改变能够引起人芽囊原虫繁殖方式的改变,出现短暂性多样化繁殖方式,人芽囊原虫对环境的适应能力很强。
Objective To understand the adaptability of Blastocystis protozoa to external conditions and to observe the effects of changes in culture conditions on its morphology and reproduction. Methods Human budding protozoa were isolated from the stool samples of diarrhea patients and cultured in locke’s egg-double phase culture medium (LES) and modified 1640 medium (RPMI-1640) respectively. The culture conditions were changed, Morphology and breeding methods. Results There were four main morphological features of H. piricota in both culture media: vacuolar, granular, amoeba and cystic. The main mode of reproduction was di-division, with a few sprouting. When the LES medium was suddenly changed to RPMI-1640 medium, the growth of H. piricota stagnated in a short time (48 ~ 72h), and then many forms of reproduction appeared. More bifidobacteria, budding, Three split, multi-split, split body reproduction, sustained 24h after the restoration of the main split. Conclusion The change of culture conditions can cause the change of reproductive methods of Blastocystis protozoa, and the emergence of transient multiplication modes. The ability of Blastocystis protozoa to adapt to the environment is very strong.