近几年高考中经常出现并且难度也较大的内容就是限制酶和DNA连接酶在识记操作中的应用技巧。而基因工程的操作水平是DNA分子水平,是肉眼无法看见的,因此给这两种工具酶的教学带来了一定的难度,我充分运用模拟实验的优势,突破工具酶教学的重难点。一、限制性核酸内切酶作用的突破限制性核酸内切酶的基本知识如来源、种类等属于识记的内容,学生在这个方面不存在理解的难题,而限制性核酸内切酶的作 In recent years, the college entrance examination often appear and more difficult content is the restriction enzyme and DNA ligase in the application of skills in the identification. The genetic engineering level of operation is the level of DNA molecules, is invisible to the naked eye, so to these two kinds of tools of teaching has brought a certain degree of difficulty, I make full use of the advantages of simulation experiments, tools and teaching breakthrough in the heavy difficulty. First, the restriction endonuclease effect Breakthrough The basic knowledge of restriction endonucleases such as the source, the type belongs to the content of the mark, the students do not exist in this area to understand the problem, and the restriction endonuclease