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目的 本研究用敏感的裸鼠为实验动物 ,裸鼠吸入肾综合征出血热病毒气溶胶后 ,进行动物感染实验研究。方法 以感染后裸鼠发病症状、体征和脑、肺组织中病毒抗原检查及组织培养及免疫组化进行肾综合征出血热病毒抗原定位为客观指标。结果 经含肾综合征出血热病毒的气溶胶吸入后 ,裸鼠 7～ 10d发病 ,感染率为 75 %。这些研究表明肾综合征出血热病毒可以气溶胶形式经呼吸道感染。结论 这对研究肾综合征出血热病毒的传播途径及肾综合征出血热防治提供了重要的理论依据。
Objective In this study, sensitive nude mice were used as experimental animals and nude mice were inoculated with haemorrhagic fever with renal syndrome virus aerosol to study the animal infection. Methods The incidence of symptoms and signs of nude mice after infection, and examination of viral antigen in brain and lung tissues, tissue culture and immunohistochemistry were used as objective indexes to identify hemorrhagic fever with renal syndrome antigens. Results After inhalation of aerosol of hemorrhagic fever with renal syndrome, nude mice developed from 7 to 10 days with an infection rate of 75%. These studies show that HSV can be transmitted through the respiratory tract as an aerosol. Conclusion This provides an important theoretical basis for the study of transmission of hemorrhagic fever with renal syndrome and prevention and treatment of hemorrhagic fever with renal syndrome.