论文部分内容阅读
目的探讨干扰素α2a对人鼻咽癌细胞株CNEI放射敏感性及其细胞周期的影响。方法用不同浓度的干扰素α2a处理CNEI细胞后用6MVX线照射,计算细胞存活率。流式细胞仪分析CNEI细胞周期动力学改变。结果0.5×106、1.0×106、1.5×106IU/L干扰素α2a干预CNEI细胞后细胞存活分数分别为0.62、0.43、0.20,放射增敏比为1.16、1.57、1.93;1×106IU/L干扰素α2a干预CNEI细胞24h后,与对照组比较细胞增殖周期G1和G2+M细胞数增多,S期细胞数减少(P<0.01)。结论干扰素α2a能抑制人鼻咽癌细胞株CNEI的生长,并具有放射增敏效应,放射增敏机制可能与其调节CNEI细胞增殖周期有关。
Objective To investigate the effects of interferon α2a on radiosensitivity and cell cycle of human nasopharyngeal carcinoma cell line CNEI. Methods The CNE1 cells were treated with different concentrations of interferon α2a and then irradiated with 6MVX line to calculate the cell viability. Analysis of cell cycle kinetics in CNEI by flow cytometry. Results After 0.5 × 106,1.0 × 106,1.5 × 106IU / L interferon α2a treatment, the cell viability of CNEI cells were 0.62,0.43,0.20, the radiosensitivity ratio was 1.16, 1.57, 1.93; 1 × 106IU / L interferon After α2a intervention for 24 hours, the number of G1 and G2 + M cells increased and the number of S phase cells decreased (P <0.01) compared with the control group. Conclusions Interferon α2a can inhibit the growth of human nasopharyngeal carcinoma cell line CNEI and has the radiosensitizing effect. The mechanism of radiosensitization may be related to its regulation of CNEI cell proliferation cycle.