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目的:CagA和VacA能否作为幽门螺杆菌(H.pylori)相关性疾病的预测因子尚有争论,本文旨在研究胃癌高发区的福州市不同胃病患者的H.prLori CagA和VacA的检出率,探讨这二者作为H.pylori毒力标志物的可行性。 方法:胃镜或手术及病理证实的慢性胃病患者170例纳入研究,胃窦癌(CC)34例,十二指肠溃疡(DU)39例,胃溃疡(GU)35例,慢性胃炎62例。无症状的健康志愿者36名为对照组,免疫印迹法检测血清中H.pylori抗体。部分患者同时接受~(14)碳-尿素呼气检测。 结果:DU组、GU组和GC组的HP血清学阳性率高于胃炎组(X~2=4.84,P=0.028)和对照组(X~2=25.877,P<0.001),DU组与GU组、GC组之间的差异无显著性意义(X~2=3.306,P=0.191)。DU组的呼气实验阳性率高于GC组(X~2=16.463,P<0.001)、胃炎组(X~2=4.31,P=0.038)和对照组(X~2=33.33,P<0.001),GU组和胃炎组高于对照组(P<0.05);而DU组与GU组之间、GC组和对照组之间的差异均无统计学意义(P=0.144)。根据免疫印迹结果进行分型,各组间CgaA、VacA和Ⅰ型菌(CagA+、VacA+)阳性率无显著性差异(P>0.05)。 结论:尽管H.pylori感染与慢性胃病有关,但血清CagA和VacA抗体对预测H.pylori感染的后果没有意义。
Aims: Whether CagA and VacA can be used as predictors of H. pylori-related diseases remains controversial. This study aimed to investigate the detection rates of H. pylori CagA and VacA in patients with different gastric diseases in Fuzhou, a high incidence area of gastric cancer , To explore the feasibility of both as virulence markers of H.pylori. Methods: One hundred and seventy patients with gastroscopy or pathologically confirmed chronic gastritis were included in the study. Gastric cancer (CC) 34, duodenal ulcer (DU) 39, gastric ulcer (GU) 35 and chronic gastritis 62. Thirty-six asymptomatic healthy volunteers were used as control group, and the serum H.pylori antibody was detected by immunoblotting. Some patients also accept ~ (14) carbon-urea breath test. Results: The positive rate of HP serology in DU group, GU group and GC group was higher than that in gastritis group (X ~ 2 = 4.84, P = 0.028) and control group (X ~ 2 = 25.877, P <0.001) There was no significant difference between GC group and GC group (X ~ 2 = 3.306, P = 0.191). The positive rate of breath test in DU group was higher than that in GC group (X 2 = 16.463, P <0.001), gastritis group (X 2 = 4.31, P = 0.038) and control group ), GU group and gastritis group were higher than those in control group (P <0.05). There was no significant difference between DU group and GU group, GC group and control group (P = 0.144). According to the results of immunoblotting, the positive rates of CgaA, VacA and Type I (CagA +, VacA +) in each group were not significantly different (P> 0.05). CONCLUSIONS: Although H.pylori infection is associated with chronic stomach problems, serum CagA and VacA antibodies are not relevant for predicting the consequences of H.pylori infection.