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菝葜是治疗盆腔炎(PID)的传统中药,其中的黄酮类化合物已被确定为抗炎的主要活性成分.然而,目前尚未从菝葜中提取出高含量的总黄酮,总黄酮的作用机制也不明确.本研究目的是提取得到菝葜中的总黄酮,并阐明其对大鼠PID的作用机制.本文首次在菝葜中获得高含量(达到55.6％)的总黄酮,分离纯化后通过NMR鉴定了其中的15个化合物.菝葜总黄酮能降低慢性盆腔炎大鼠子宫组织中IL-6和IL-1β的水平,这可能是菝葜活性成份治疗慢性盆腔炎症和缓解盆腔粘连的药理作用机制之一.“,”As a Chinese medicinal plant,Smilax china is traditionally used in the treatment of pelvic inflammatory disease(PID).Flavonoids have been identified as the anti-inflammatory bioactive fraction.However,the high content of total flavonoids has not been extracted from S.china.The therapeutic effects and mechanisms of total flavonoids are still unknown.In the present study,we aimed to obtain the effective parts of flavonoids in S.china,and elucidate the mechanism of action on PID.The anti-inflammatory effect was evaluated on phenol mucilage-induced pelvic inflammation in rats.This was the first time that total flavonoids in high concentration(up to 55.6％)were obtained in S.china.A total of 15 compounds were separated and identified by NMR,and the total flavonoids were detected with HPLC.TFSC caused a reduction in serum levels of IL-6 and IL-1β.These results suggested that TFSC had a significant anti-PID effect,probably due to inhibition of the inflammatory reaction.