论文部分内容阅读
为了解染色体易位对小麦农艺性状的影响,以豫农982(1BL/1RS易位系)和wheatear(7DL.7Ag易位系)杂交后代的900个F2群体及其F2∶3家系为实验材料,对F2群体进行1BL/1RS易位和7DL.7Ag易位类型的分子检测,并对F2群体及F2∶3家系的主要农艺性状进行田间调查(F2群体的农艺性状仅作参考,重点分析F2∶3家系的农艺性状)。结果表明,(1)STS标记Lr19130与SSR引物Xgwm428联合使用可作为共显性标记鉴定纯合与杂合的7DL.7Ag易位,完善了7DL.7Ag易位的分子检测方法;(2)1BL/1RS易位可显著降低株高,提高穗粒数与小穗数;(3)7DL.7Ag易位在籽粒千粒重和饱满度上有显著的正向作用,但7DL.7Ag易位的穗粒数显著低于非7DL.7Ag易位,且有延迟小麦成熟和增加株高的趋势;(4)1BL/1RS和7DL.7Ag双重易位可同时提升小穗数和千粒重,但穗粒数减少。
In order to understand the effect of chromosomal translocation on the agronomic traits of wheat, 900 F2 populations and their F2: 3 pedigrees were crossed with the crosses between the two crosses of Yuongnong 982 (1BL / 1RS) and wheatear (7DL.7Ag) , The F2 population and 1DL / 1RS translocation and the 7DL.7Ag translocation type were detected by molecular marker. The main agronomic traits of F2 population and F2: 3 were investigated in field (F2 population agronomic traits were only used as a reference, focusing on F2 : 3 pedigree agronomic traits). The results showed that: (1) The combination of STS marker Lr19130 and SSR primer Xgwm428 could be used as a co-dominant marker to identify homozygous and heterozygous 7DL.7Ag translocations and perfect the molecular detection method of 7DL.7Ag translocation; (2) 1BL / 1RS translocation significantly reduced the plant height and increased the number of spikelets and spikelets. (3) The 7DL.7Ag translocation had a significant positive effect on 1000-grain weight and plumpness, but 7DL.7Ag translocation (4) The double translocation of 1BL / 1RS and 7DL.7Ag could increase spikelet number and 1000-grain weight at the same time, but the number of grains per spike was reduced .