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在确定5种泡桐叶片诱导愈伤组织基本培养基为MS的基础上,通过不同的NAA和BA的组合,筛选出了毛泡桐(Paulownia tomentosa)、南方泡桐(Pauiownia australis)、白花泡桐(Paulownia fortunei)、兰考泡桐(Paulownia elongata)和豫杂一号泡桐(P.tomentosa x P. fortunei)叶片愈伤组织诱导、芽分化和根分化的最适培养基。MS+0.5NAA+4BA、MS+0.3NAA+2BA、MS+0.5NAA+4BA、MS+0.3NAA+6BA和MS+0.3NAA+8BA分别为五种泡桐树种叶片愈伤组织诱导的最适培养基,上述树种的叶片愈伤组织诱导芽分化最适培养基分别为MS+0.3NAA+12 BA、MS+0.3NAA+12 BA、MS+0.5NAA+12 BA、MS+0.5NAA+12 BA和MS+0.7NAA+12 BA,最后找出了5种泡桐芽诱导根的最适培养基分别为1/2MS+0.1NAA、1/2MS+0.1NAA、1/2MS、1/2MS+0.3NAA和1/2MS+0.5NAA。这些结果为开展泡桐基因工程研究和利用不同种泡桐叶片原生质体融合培育泡桐新品种提供了参考。
On the basis of determining the basic medium for callus induction of five kinds of Paulownia leaves, we selected Paulownia tomentosa, Pauiownia australis, Paulownia fortunei ), Paulownia elongata and P. tomentosa x P. fortunei, respectively. The optimum medium for the induction, bud differentiation and root differentiation of callus were studied. MS + 0.5NAA + 4BA, MS + 0.3NAA + 2BA, MS + 0.5NAA + 4BA, MS + 0.3NAA + 6BA and MS + 0.3NAA + 8BA were the optimal medium for leaf callus induction of five species of Paulownia trees , The optimum mediums for bud differentiation of leaf callus induction of the above species were MS + 0.3NAA + 12BA, MS + 0.3NAA + 12BA, MS + 0.5NAA + 12BA, MS + 0.5NAA + 12BA and MS + 0.7 NAA + 12 BA. Finally, the optimal culture medium for the induction of five kinds of Paulownia buds was 1 / 2MS + 0.1NAA, 1 / 2MS + 0.1NAA, 1 / 2MS, 1 / 2MS + 0.3NAA and 1 /2MS+0.5NAA. These results provide a reference for the genetic engineering of Paulownia and the use of different varieties of Paulownia leaf protoplast fusion Paulownia new varieties.