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香茶菜提取物黄酮体外对人肝癌细胞系GHC-3的诱导,从细胞生长特性、膜受体、肿瘤标志物、癌基因产物和遗传性状等方面观察到细胞恶性表型的逆转。经过药物作用10周后发现细胞形态由多边形上皮样逐渐转变为梭形,生长速度减慢,分裂指数、集落形成、ConA凝集和ConA受体、DNA含量及~3H-TdR掺入均明显减少,染色体分布较集中,出现二倍体细胞等变化。进一步以三种单克隆抗体测试,AFP和ras P21合成以及荧光标志抗体显示的膜抗原都明显减弱。这些改变在脱离药物后一个月以上仍维持稳定。以上结果表明,香茶菜黄酮可诱导人肝癌细胞改变恶性表型特征,促使向正常或降低恶性方向逆转。
Induction of human hepatocellular carcinoma cell line GHC-3 by flavonoids of Sabina vulgaris in vitro, the reversal of the malignant phenotype was observed from the aspects of cell growth characteristics, membrane receptors, tumor markers, oncogene products, and hereditary traits. After 10 weeks of drug treatment, the cell morphology gradually changed from polygonal epithelium to spindle, the growth rate slowed, mitotic index, colony formation, ConA agglutination, and ConA receptor, DNA content and ~3H-TdR incorporation were significantly reduced. Chromosomal distribution is more concentrated and there are diploid cells and other changes. Further testing with three monoclonal antibodies, AFP and ras P21 synthesis and fluorescent marker antibodies showed significant reduction in membrane antigens. These changes remained stable for more than a month after leaving the drug. The above results indicate that the flavonoids of Xiangcai can induce the change of malignant phenotypes of human hepatocellular carcinoma cells and promote the reversion to the normal or reduced malignant direction.