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目的：了解粒细胞巨噬细胞集落刺激因子（ＧＭＣＳＦ）对人卵巢癌细胞株生长的影响。方法：采用ＸＴＴ比色法及流式细胞仪检测ＧＭＣＳＦ对卵巢癌细胞株ＳＫＯＶ３、３ＡＯ及ＣＡＯＶ３ 细胞生长的影响。结果：用ＸＴＴ法检测ＧＭＣＳＦ对ＳＫＯＶ３、３ＡＯ 及ＣＡＯＶ３ 细胞的生长均无明显影响；流式细胞仪检测也未发现ＧＭＣＳＦ对ＳＫＯＶ３、３ＡＯ及ＣＡＯＶ３细胞的增殖指数有明显影响。结论：在卵巢癌化疗过程中应用ＧＭＣＳＦ是比较安全的，对其潜在的促进肿瘤细胞增殖的顾虑也许是不必要的。
Objective: To investigate the effect of granulocyte-macrophage colony-stimulating factor (GMCSF) on the growth of human ovarian cancer cell lines. Methods: The effects of GMCSF on the growth of ovarian cancer cell lines SKOV3,3AO and CAOV3 were detected by XTT assay and flow cytometry. Results: The results of XTT assay showed that GM-CSF had no significant effect on the growth of SKOV3, 3AO and CAOV3 cells. Flow cytometry also did not find any significant effect of GM-CSF on the proliferation index of SKOV3, 3AO and CAOV3 cells. Conclusion: The application of GMCSF in ovarian cancer chemotherapy is safer and may not be necessary for its potential to promote tumor cell proliferation.