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应用放射配基结合测定法,以R1881为特异配基;分析正常成年男性雄激素靶组织─—外阴皮肤组织中细胞质和核基质雄激素受体(AR),同时分别用Scatchard、woolf和双倒数作图法三种常用数据处理方法详细分析AR的Bmax(Bm)和Kd。结果表明,作为阳性对照和质量控制的6例雄性大鼠肝脏细胞质AR的Bm为4.77±0.61pmol/g蛋白,Kd为9.662.03×10-10mol/L。11例正常男性的细胞质ARBm为24.03±7.19pmol/g蛋白,Kd为15.13±7.06×10-10mol/L;细胞核内AR的Bm为204.37±119.62pmol/g蛋白,Kd为6.38±4.14×10-10mol/L。Scatchard、Woolf和双倒数法分析的结果均一致。提示AR的Scatchard作图分析是稳定可靠的,并且具有显著的实用价值。
Radioligand binding assay and R1881 were used as specific ligands. Cytoplasm and nuclear matrix androgen receptor (AR) in normal adult male androgen target tissues and vulvar skin tissues were analyzed. Scatchard, woolf and double countdown Three commonly used methods of data processing methods for the analysis of Bmax (Bm) and Kd AR. The results showed that the Bm of cytoplasm in the cytoplasm of 6 male rats as positive control and quality control was 4.77 ± 0.61 pmol / g protein, Kd was 9.662.03 × 10-10 mol / L. The cytoplasmic ARBm of 11 normal men was 24.03 ± 7.19 pmol / g protein, Kd was 15.13 ± 7.06 × 10-10 mol / L; the Bm of AR in nucleus was 204.37 ± 119.62 pmol / g protein, Kd was 6.38 ± 4.14 × 10-10 mol / L. The results of Scatchard, Woolf and double reciprocal analysis are consistent. It is suggested that the Scatchard plot analysis of AR is stable and reliable and has significant practical value.