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P_(53)基因位于17号染色体(17P13),涉及细胞增殖的调控。作者于66例急性髓性白血病(AML)患者采用单链构型多态分析法(SSCP)测定外显子5—8的突变率及其特点,并以序列分析予以确定。方法66例患者白血病细胞数均>50%,骨髓细胞经24小时培养后进行染色体R 带和G 带分析。PCR-SSCP 分析:2个基因组被扩增,1区含外显
The P_(53) gene is located on chromosome 17 (17P13) and is involved in the regulation of cell proliferation. The authors in 66 cases of acute myeloid leukemia (AML) patients using single-stranded configuration polymorphism analysis (SSCP) to determine the mutation rate and characteristics of exon 5-8, and to determine the sequence. Methods The number of leukemic cells in 66 patients was more than 50%. The chromosome R band and G band analysis were performed after bone marrow cells were cultured for 24 hours. PCR-SSCP analysis: Two genomes were amplified and one region contained foreign