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大岩桐,叶片浓绿厚实,花朵大,花期长,是优秀的温室盆栽花卉之一。通过组织培养,大岩桐可以实现工厂化生产。组培快繁要点1.外植体的处理:剪取新生叶片,用洗衣粉漂洗数次,在自来水下冲洗1～2小时,置超净工作台下用70%的酒精溶液浸10秒,再用无菌水冲洗数次,并用0.1%的升汞消毒处理6～8分钟,无菌水冲洗3～5次,最后用消毒滤纸吸干水分。2.诱导分化:将叶片切成1平方厘米的小块,接入培养基 MS+BA2.0+NAA0.2中,2周后外植体膨大转绿,4周后诱导分化出白色不定芽,白色不定芽逐渐成长变绿。待不定芽长至1厘米时,切取转接培养基 MS+BA0.5中,1～2周时基部腋芽萌动,形成芽丛,4周可继代增殖一次,繁殖系数可达6～8。切分1.5～2厘米高的无根苗转接人培养基1/2MS 中,3～4周基部形成小球茎,并长出4～5条须根。
Ophiopogon, leaves thick green thick, large flowers, long flowering period, is one of the best greenhouse potted flowers. Through tissue culture, Dayan Tong can achieve factory production. Tissue culture multiplication points 1. explant processing: cutting new leaves, rinsed with detergent several times, rinsed under running water for 1 to 2 hours, set clean bench with 70% alcohol solution soaked for 10 seconds, Then rinse with sterile water several times and disinfected with 0.1% mercuric chloride 6 to 8 minutes, rinsed with sterile water 3 to 5 times, and finally with a sterile filter paper to dry the water. Induction of differentiation: The leaves were cut into small pieces of 1 cm square, and the medium was inserted into the medium MS + BA2.0 + NAA0.2. After 2 weeks, the explants expanded to turn green and differentiated into white adventitious buds after 4 weeks , White adventitious buds grow green gradually. To be adventitious buds grow to 1 cm long, cut the transfer medium MS + BA0.5, 1 to 2 weeks base axillary buds germinate to form bud plexus, 4 weeks can be subculture once, the breeding coefficient of up to 6 ~ 8. Cut 1.5 ~ 2 cm high rootless seedlings transferred to human medium 1 / 2MS, 3 to 4 weeks at the base of the formation of small bulbs, and grow 4 to 5 fibrous roots.