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目的:探讨运动预适应(EP)对异丙肾上腺素(ISO)导致的大鼠急性心肌缺血损伤的早期保护作用。方法:健康雄性SD大鼠52只,随机分为对照组(C组)、运动预适应组(EP组)、异丙肾上腺素组(ISO组)、运动预适应+异丙肾上腺素组(EP+ISO组)。在EP模型的基础上,用大剂量ISO复制大鼠急性心肌缺血损伤模型,采用HE染色、HBFP染色和计算机图像分析技术显示大鼠心肌缺血缺氧的改变,用免疫化学发光法检测大鼠血清心肌肌钙蛋白I(cTnl)的含量。结果:(1)与C组相比,EP组HE染色均匀,轮廓清晰,HBFP染色未见缺血缺氧改变;血清cTnl升高,但无统计学意义(P>0.05)。(2)与C组相比,ISO组HE染色显示心肌细胞肿胀红染,细胞界限模糊,HBFP染色可见明显的缺血缺氧改变;血清cTnl升高显著(P<0.05)。(3)与ISO组相比,EP+ISO组心肌缺血缺氧改变较轻,缺血缺氧面积和平均光密度明显减少(P<0.05);血清cTnl明显降低(P<0.05),但仍显著高于C组(P<0.05)。结论:EP诱导机体产生了缺血预适应的保护效应,能够减轻ISO导致的急性心肌缺血损伤的程度,对急性心肌缺血损伤具有一定的保护作用。
Objective: To investigate the early protective effect of exercise preconditioning (EP) on acute myocardial ischemia induced by isoproterenol (ISO) in rats. Methods: Fifty-two healthy male SD rats were randomly divided into control group (C group), exercise preconditioning group (EP group), isoproterenol group (ISO group), preconditioning + isoproterenol group + ISO group). On the basis of the EP model, a large dose of ISO was used to duplicate the model of acute myocardial ischemia in rats. HE staining, HBFP staining and computer image analysis were used to detect the changes of myocardial ischemia and hypoxia in rats. The immunochemical chemiluminescence Serum cardiac troponin I (cTnl) content. Results: (1) Compared with group C, the EP group was homogenized with HE and the outline was clear. There was no change of hypoxia and hypoxia in HBFP staining. The level of cTnl in serum increased but there was no statistical significance (P> 0.05). (2) Compared with group C, the HE staining of ISO group showed that the myocardial cells were swollen and stained, the cell boundaries were fuzzy, and obvious hypoxia and hypoxia changes were observed by HBFP staining. Serum cTnl level increased significantly (P <0.05). (3) Compared with ISO group, the changes of myocardial ischemia and hypoxia in EP + ISO group were lighter, the areas of ischemia and hypoxia and the average optical density were significantly decreased (P <0.05), while the serum cTnl was significantly lower (P <0.05) Still significantly higher than C group (P <0.05). CONCLUSION: EP induces the protective effect of ischemic preconditioning on the body and can reduce the extent of acute myocardial ischemia induced by ISO, which has a protective effect on acute myocardial ischemia.