“分泌蛋白”是指由活细胞所分泌的丰富的、具有复杂生理功能的一系列蛋白，包括细胞因子、趋化因子、激素、消化酶、抗体、胞外蛋白酶和毒素等，这些蛋白参与细胞信号转导、粘连、迁移和免疫防御等多种生物过程，发挥重要的生理作用。近年来，蛋白组学技术飞速发展，极大地促进了分泌蛋白组学的研究和发展。分泌蛋白组学已经被广泛应用于人类生殖领域的探索，并鉴定了许多蛋白质，这些蛋白质可能是人类辅助生殖技术成功妊娠的潜在生物标志物或治疗目标。本文综述了分泌蛋白组学在人卵泡液、胚胎发育及子宫内膜分泌物中的研究进展，为分泌蛋白组学在人类生殖领域研究中的深入应用提供借鉴。“,”“Secreted protein” refers to a series of proteins with complex physiological functions secreted by living cells, including cytokines, chemokines, hormones, digestive enzymes, antibodies, extracellular proteases and toxins. These proteins play important physiological roles in a variety of biological processes, such as cell signal transduction, adhesion, migration and immune defense. In recent years, the rapid development of proteomics technology has greatly promoted the research of secretomics. Secretomics has been widely used in the field of human reproduction, and many proteins have been identified, which may be potential biomarkers or therapeutic targets for successful pregnancy in human assisted reproductive techniques. In this paper, the research progress of secretomics in human follicular fluid, embryonic development and endometrial secretions were reviewed in order to provide reference for the further application of secretomics in the field of human reproduction.