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目的:观察蒙药匝迪-5对CUM大鼠海马、前额叶皮质单胺递质的影响,探讨其作用机制。方法:将60只SD雄性大鼠按体质量随机分为正常组、模型组、西药组和蒙药低、中、高剂量组,每组10只。采用慢性不可预见性应激结合孤养的方法造模,以体质量变化、Open-field实验进行行为学评价及酶联免疫法(ELISA)检测大鼠海马和前额叶皮质5-HT、NE、DA的含量。结果:与正常组相比,模型组大鼠体质量增长缓慢,Open-Field实验水平活动得分和垂直活动得分降低,海马和前额叶皮质组织5-HT、DA及NE的含量显著降低(P<0.05,P<0.01)。与模型组相比,西药组和蒙药各剂量组可不同程度改善模型大鼠行为改变,其中氟西汀和匝迪-5高、中剂量可不同程度提高模型大鼠海马和前额叶皮质5-HT、DA、NE的含量(P<0.05,P<0.01),低剂量作用不明显。结论:匝迪-5的抗抑郁机制可能通过提高脑内5-HT、NE、DA的含量发挥作用。
OBJECTIVE: To observe the effect of Mongodiol-5 on the monoamine neurotransmitter in hippocampus and prefrontal cortex of CUM rats and to explore its mechanism. Methods: Sixty SD male rats were randomly divided into normal group, model group, western medicine group and Mongolian medicine low, medium and high dose groups, with 10 rats in each group. Chronic unpredictable stress combined with solitary method was used to establish models. The behavioral evaluation and enzyme-linked immunosorbent assay (ELISA) were used to detect the changes of 5-HT, NE, DA content. Results: Compared with the normal control group, the body mass of rats in model group increased slowly, the scores of Open-Field and vertical activities decreased, and the content of 5-HT, DA and NE in hippocampus and prefrontal cortex decreased significantly (P < 0.05, P <0.01). Compared with the model group, the western medicine group and the Mongolian medicine each dose group can improve the behavioral changes of the model rats to varying degrees, of which fluoxetine and resorcin-5 high and medium doses can improve the hippocampus and prefrontal cortex of the rat model rat 5- HT, DA, NE content (P <0.05, P <0.01), low-dose effect was not obvious. Conclusion: The antidepressant mechanism of ZD-5 may play an important role in increasing brain 5-HT, NE and DA levels.