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目的:探讨EGCG体外诱导胃癌MGC-803细胞凋亡的作用。方法:以不同浓度EGCG分别处理MGC-803细胞,用琼脂糖凝胶电泳、电镜、MTT法观察MGC-803细胞DNA带型、形态和抑制率的变化。结果:琼脂糖凝胶电泳呈典型的DNA“梯状带”,但高浓度的EGCG(1×10~(-3)mol/L)作用MGC-803细胞33小时后,电泳显示细胞坏死后模糊的DNA呈弥散的“膜状带”。电镜观察可见染色质凝集呈新月状附在核膜周边,细胞变成数个大小不等的由膜包裹的凋亡小体。MTT法测定1×10~(-3)mol/L、1×10~(-4)mol/L、1×10~(-5)mol/L、1×10~(-6)mol/L、1×10~(-7)mol/L EGCG对MGC-803细胞生长抑制率分别为68.39%、20.38%、20.30%、16.99%、13.22%。结论:EGCG有明显的诱导胃癌MGC-803细胞凋亡作用,高浓度的EGCG直接导致细胞坏死。
Objective: To investigate the effect of EGCG on inducing apoptosis of gastric cancer MGC-803 cells in vitro. Methods: MGC-803 cells were treated with different concentration of EGCG. The changes of DNA band type, morphology and inhibition rate of MGC-803 cells were observed by agarose gel electrophoresis, electron microscopy and MTT assay. RESULTS: Agarose gel electrophoresis was a typical DNA “ladder”, but after high concentration of EGCG (1 × 10 -3 mol/L) was applied to MGC-803 cells for 33 hours, electrophoresis showed that the cells were necrotic and fuzzy. The DNA is a diffuse “membrane band.” Electron microscopy showed that the chromatin condensation was in the form of a crescent moon attached to the periphery of the nuclear membrane, and the cells became several apoptotic bodies surrounded by membranes of different sizes. The MTT method was used to determine 1×10 -3 mol/L, 1×10 -4 mol/L, 1×10 -5 mol/L and 1×10 -6 mol/L. The inhibition rates of 1×10 -7 mol/L EGCG on MGC-803 cells were 68.39%, 20.38%, 20.30%, 16.99%, and 13.22%, respectively. Conclusion: EGCG has obvious apoptosis-inducing effect on gastric cancer MGC-803 cells, and high concentration of EGCG directly leads to cell necrosis.