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目的探讨蜂毒肽抗风湿疼作用及其机制。方法取SD大鼠40只,随机分为空白对照组、蜂毒组、蜂毒肽组、模型对照组。蜂毒组、蜂毒肽组、模型对照组以完全弗氏佐剂诱导拓关节类风湿关节炎模型,空白对照组不诱导模型,为对照;空白对照组、蜂毒组、蜂毒肽组、模型对照组于足三里分别注射生理盐水、蜂毒、蜂毒肽和生理盐水;测量关节肿胀度、缩足逃避反射时间。结果造模时各组拓关节体积、缩足逃避反射时间基本一致。方差分析分析显示造模4 d后,蜂毒组、蜂毒肽组、模型对照组与空白对照组拓关节厚度差异均有显著性;8 d后模型组拓关节体积均有降低趋势,以蜂毒组、蜂毒肽组组更明显,与空白对照组、模型对照组差异均有显著性。蜂毒组、蜂毒肽组缩足逃避反射时间在各个时间点均长于空白对照组、模型对照组,且差异均有显著性;而蜂毒组、蜂毒肽组间差异无统计学意义。结论蜂毒肽可能是蜂毒治疗类风湿性关节炎的主要成分。
Objective To investigate the anti-rheumatic pain effect of melittin and its mechanism. Methods Forty SD rats were randomly divided into blank control group, melittin group, melittin group and model control group. Melittin group, melittin group, the model control group to complete Freund’s adjuvant induced rheumatoid arthritis model extension, the blank control group did not induce the model as a control; blank control group, the venom venom group, melittin group, The model control group were injected with saline, bee venom, melittin and normal saline respectively in Zusanli. The swelling degree of joint and the evading reflex time were measured. Results When modeling the size of each extension Tuojiao, reduced foot evasion reflection time is basically the same. Analysis of variance (ANOVA) showed that there were significant differences in topological thickness between the bee venom group, the melittin group, the model control group and the blank control group after 4 days of modeling. At 8 days, The toxicity group and the melittin group were more obvious, and the differences were significant with the blank control group and the model control group. The evoked reflex time of the venom of the bee venom and melittin groups was longer than that of the blank control group and the model control group at each time point, and the difference was significant. However, there was no significant difference between the venom and the melittin groups. Conclusion Melittin may be the main component of bee venom in the treatment of rheumatoid arthritis.