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建立HPLC法测定兔组织中荭草苷含量的方法,研究静脉给药时,荭草苷在新西兰白兔体内组织中的分布特点,荭草苷给药后,分时采集组织样品,放血处死动物,取出心、肝、脑、肾、肺、脾组织并制备匀浆,以HPLC法测定兔组织中荭草苷含量,采用统计矩理论计算各个组织的AUC。组织中的内源性物质不干扰荭草苷的测定,在0.050 0～50.0mg/L范围内,荭草苷质量浓度和峰面积线性关系良好,定量下限为0.050 0mg/L。精密度、准确度和加样回收率RSD值均小于15%。各组织的AUC顺序为肾>肝>肺>脾>心>脑。将各组织AUC进行对数转换后,依次进行正态性检验、方差齐性检验和方差分析,结果显示,不同组织的AUC有显著性差异(p<0.05),荭草苷在新西兰白兔体内主要组织中的含量分布有明显不同。
To establish a HPLC method for the determination of toxin in rabbit tissue, to study the distribution characteristics of orientin in vivo in New Zealand white rabbits after intravenous administration. After the administration of orientin, the tissue samples were collected at different times, and the animals were sacrificed The tissues of heart, liver, brain, kidney, lung and spleen were removed and homogenized. The content of toxin in rabbit tissues was determined by HPLC. The AUC of each tissue was calculated by statistical moment theory. The endogenous substances in the tissue did not interfere with the determination of orientin. The linearity between the concentration of orientin and the peak area was good in the range of 0.050 0-50.0 mg / L with the lower limit of quantification being 0.050 mg / L. RSD precision, accuracy and sample recovery were less than 15%. The AUC order of each tissue was kidney> liver> lung> spleen> heart> brain. The AUC of each tissue was logarithmically transformed, followed by normality test, homogeneity of variance test and analysis of variance. The results showed that the AUC of different tissues was significantly different (p <0.05) The content distribution in major organizations is significantly different.