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随着基于VP1序列分析的分子定型方法的推广应用,许多新型人类肠道病毒(Human enterovirus,HEV)陆续被发现。本研究通过对1989～2010年山东省急性弛缓性麻痹(Acute flaccid paralysis,AFP)监测系统分离到的非脊髓灰质炎肠道病毒进行VP1完整编码区的序列扩增及分子定型,共发现1株EV74,3株EV80和1株EV87。同源性分析显示EV74、EV80和EV87山东分离株与原型株的核苷酸同源性分别为81.4%,76.4%～81.7%以及80.3%。系统发生学分析显示山东地方株与国内外其他分离株的亲缘关系均较远。这是在中国大陆地区首次发现EV74和EV87,其中EV87山东分离株为全球第2个鉴定出的分离株。这3种新型HEV在AFP监测系统中的分离率较低,提示尚未在我国导致大规模流行。
With the popularization and application of molecular typing based on VP1 sequence analysis, many new human enterovirus (HEV) have been discovered. In this study, we sequenced and sequenced the VP1 complete coding region of non-polio enterovirus isolated from the acute flaccid paralysis (AFP) surveillance system in Shandong province from 1989 to 2010. A total of 1 strains were found EV74, 3 strains of EV80 and 1 strain of EV87. Homology analysis showed that the nucleotide homology between the Shandong isolate and the prototype strain of EV74, EV80 and EV87 were 81.4%, 76.4% -81.7% and 80.3%, respectively. Phylogenetic analysis showed that the genetic relationship between Shandong local strains and other isolates at home and abroad were far. This is the first discovery of EV74 and EV87 in mainland China, of which EV87 Shandong isolate is the second one identified in the world. The isolation rates of these three new HEVs in the AFP monitoring system are low, suggesting that they have not yet led to large-scale epidemic in our country.