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目的:探讨胃癌中雌激素受体(ER)-α36与ER-α66的mRNA表达及其临床病理意义。方法:采用实时荧光定量RT-PCR方法检测4株胃癌细胞株中ER-α36和ER-α66 mRNA的表达情况;采用半定量RT-PCR方法检测20例人胃腺癌组织中肿块、癌旁组织及正常组织中的ER-α36和ER-α66 mRNA表达情况。结果:ER-α36和ER-α66 mRNA在胃癌细胞株和人体胃癌样本中均有表达。胃癌细胞株中ER-α36表达量高于ER-α66;人体样本中ER-α66在正常组织表达量最高,ER-α36在癌组织中表达量最高,且与患者的年龄、肿瘤的大小、组织学分级以及淋巴结转移相关。结论:ER-α36在胃癌细胞中发挥的作用可能大于ER-α66,ER-α36 mRNA的高表达与胃癌的生物学行为相关。
Objective: To investigate the mRNA expression of estrogen receptor (ER) -α36 and ER-α66 in gastric cancer and its clinicopathological significance. Methods: The mRNA expression of ER-α36 and ER-α66 in 4 gastric cancer cell lines was detected by real-time fluorescence quantitative RT-PCR. The expression of ER-α36 and ER-α66 mRNA in 20 gastric adenocarcinoma tissues were detected by semi-quantitative RT- The expression of ER-α36 and ER-α66 mRNA in normal tissues. Results: ER-α36 and ER-α66 mRNA were expressed in both gastric cancer cell lines and human gastric cancer samples. The expression of ER-α36 in gastric cancer cell lines was higher than that of ER-α66. The expression of ER-α66 in human samples was the highest, while the expression of ER-α36 was the highest in cancer tissues. The expression of ER-α36 was correlated with age, tumor size, Learning grade and lymph node metastasis. CONCLUSION: ER-α36 may play a more important role in gastric cancer than ER-α66. The high expression of ER-α36 mRNA may be related to the biological behavior of gastric cancer.