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[目的]研究紫鸭跖草在铜胁迫下表达的特异性蛋白质,以期探明紫鸭跖草耐铜机理。[方法]以超积累铜植物紫鸭跖草(Setcreasea pur-purea Boom)为试验材料,通过水培、电泳和层析等方法,分析其表达的特异性蛋白质分子量、表达时间以及表达时铜的最低浓度,同时对该特异性蛋白质进行了分离纯化。[结果]铜胁迫下紫鸭跖草诱导表达特异性蛋白质的最低铜浓度为 50 μmol/L,表达的时间是第 4 周,其分子量为 89.4kDa。[结论]该研究结果表明紫鸭跖草耐铜特性与其表达的特异性蛋白质密切相关。
[Objective] The aim of the study was to study the specific protein expressed in the plant of Brassica juncea under copper stress in order to find out the mechanism of copper tolerance. [Method] With Setcreasea pur-purea Boom as test material, the molecular weight of specific protein, expression time and expression of copper during expression were analyzed by hydroponics, electrophoresis and chromatography The lowest concentration, while the specificity of the protein was isolated and purified. [Result] The lowest copper concentration induced by the copper stress was 115 μmol / L and the expression time was 4 weeks, the molecular weight was 89.4 kDa. [Conclusion] The results of this study show that the resistance of Brassica juncea to copper is closely related to the expression of specific proteins.