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为了探讨肿瘤原位转染共刺激分子Ｂ７－１对小鼠肝癌的治疗作用及其机理，本文在建立了小鼠肝癌模型的基础上，采用表达小鼠Ｂ７－１的重组腺病毒直接瘤体内注射，结果可显著抑制小鼠肝癌的生长，明显延长荷瘤小鼠的存活期。肝癌组织ＨＥ染色及免疫组织化学分析发现，经重组腺病毒ｍＢ７－１治疗的肿瘤内大片组织坏死出血，坏死组织与未坏死组织交界处有大量淋巴细胞浸润，浸润淋巴细胞表现为ＣＤ３＋，ＣＤ８＋，ＦａｓＬ＋和ｐｅｒｆｏｒｉｎ＋。ＲＴ－ＰＣＲ显示，ＦａｓＬ和穿孔素ｍＲＮＡ表达呈阳性，进一步证实该浸润淋巴细胞呈激活状态
In order to investigate the effect and mechanism of tumor in situ transfection of co-stimulatory molecule B7-1 on hepatoma in mice, this study established a mouse liver cancer model and used recombinant mouse adenovirus expressing mouse B7-1 as a direct tumor. Injection, the results can significantly inhibit the growth of mouse liver cancer, significantly prolong the survival of tumor-bearing mice. Histological and immunohistochemical analysis of hepatocellular carcinoma showed that necrotic hemorrhage occurred in large tumor tissues treated with recombinant adenovirus mB7-1, and a large number of lymphocytes infiltrated at the junction of necrotic tissue and necrotic tissue. The infiltrating lymphocytes expressed CD3+ and CD8+. FasL+ and perforin+. RT-PCR showed positive expression of FasL and perforin mRNA, further confirming that the infiltrating lymphocytes were activated