论文部分内容阅读
近年来,由于胎儿血标本的制备和胎儿红细胞珠蛋白链合成分析的开展,使遗传性Hb病的出生前诊断成为可能。本文报道了2对夫妇有β-地中海贫血(β-地贫)危险而作出生前诊断的结果。这两例均是在遗传咨询后要求作出生前诊断的。两对夫妇中例|为β°-地贫,例2为β~+地贫,母方血型为O型,父方为AB型。胎儿血标本分别在妊娠22和21周经超声波定泣后采取。然后从母亲-胎儿的混合血中清除母亲血。例1的20μl血标本中,胎儿血占10％,例2的16,18和和19μl血标本中,胎儿血分别占40、7、10％。经试验表明:新生儿标本中红细胞低于5％时就能与抗-AB血清反应,获得令人满意的凝集作用,而且不改变β/γ比值。在本试验的2例中,例1的β/γ比值为0.038,
In recent years, prenatal diagnosis of hereditary Hb disease has become possible due to the preparation of fetal blood samples and analysis of fetal erythrocyte globin chain synthesis. This article reports the results of prenatal diagnosis of the risk of β-thalassemia (β-thalassemia) in 2 couples. Both of these requests require prenatal diagnosis after genetic counseling. In both cases, the case was β ° - thalassemia. Case 2 was β ~ + thalassemia. The maternal blood type was O type and the father type was AB type. Fetal blood samples were taken at 22 and 21 gestational weeks after sonicatorning. The mother’s blood is then removed from the maternal-fetal mixture. Fetal blood accounted for 10% of the 20 μl of the blood sample of Example 1, and fetal blood accounted for 40, 7 and 10% of the 16, 18 and 19 μl of the blood sample of Example 2, respectively. Experiments show that: Neonatal red blood cells in less than 5% can react with anti-AB serum to obtain satisfactory agglutination, and does not change the β / γ ratio. In the two cases of this experiment, the β / γ ratio of Example 1 was 0.038,