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为探讨全硫代修饰的端粒酶反义寡核苷酸(ASODN-t)抑制人膀胱癌 T24细胞增殖并诱导其凋亡的可能性,采用光镜、电镜、四唑盐(MTT)比色法及流式细胞仪等检测不同浓度 ASODN-t 对 T24细胞生长、细胞周期和诱导凋亡的作用。结果表明,不同浓度 ASODN-t 对 T24细胞生长有抑制作用,且具有序列特异性及剂量依赖性。6μmol/L ASODN-t 作用细胞72 h,电镜观察可见典型凋亡特征,流式细胞仪检测到凋亡峰,凋亡率为10.4%,而无关寡核苷酸(N-ODN)片段则无此作用。结论:ASODN-t 对人膀胱癌T24细胞生长有抑制作用,并可诱导其发生凋亡,进一步说明端粒酶与 T24细胞恶性增殖有关,抑制端粒酶活性可能成为膀胱癌基因治疗的新靶点。
To explore the possibility of ASODN-t inhibiting the proliferation and inducing apoptosis of human bladder cancer T24 cells, the effects of ASODN-t on the proliferation of human bladder cancer T24 cells were observed by light microscopy, electron microscopy, MTT assay Colorimetric method and flow cytometry were used to detect the effect of different concentrations of ASODN-t on T24 cell growth, cell cycle and apoptosis. The results showed that different concentrations of ASODN-t on T24 cell growth inhibition, and has a sequence-specific and dose-dependent. The apoptosis of ASODN-t cells treated with 6μmol / L ASODN-t for 72 hours was observed by electron microscope. The apoptotic peak was detected by flow cytometry and the apoptosis rate was 10.4%, while no N-ODN fragments This role. CONCLUSION: ASODN-t inhibits the growth of human bladder cancer T24 cells and induces its apoptosis, which further indicates that telomerase is involved in the malignant proliferation of T24 cells and inhibition of telomerase activity may be a new target of gene therapy for bladder cancer point.