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目的验证采用预真空高温高压蒸汽对骨科手术包和妇科手术包的灭菌效果。方法将大肠埃希菌、枯草芽胞杆菌黑色变种芽胞、金黄色葡萄球菌和蜡样芽胞杆菌作为指示菌,包裹于手术包内进行预真空高温高压蒸汽灭菌处理,以此来验证预真空高温高压蒸汽灭菌的灭菌效果。结果预真空高温高压蒸汽灭菌对妇科手术包中4种试验菌株灭菌率为100.0%,对骨科手术包中的大肠埃希菌、枯草芽胞杆菌黑色变种芽胞和金黄色葡萄球菌的灭菌率均为100.0%,对骨科手术包中的蜡样芽胞杆菌灭菌率为85.7%。结论预真空高温高压蒸汽灭菌能够满足绝大部分手术包的灭菌要求,但是在实际应用过程中,需要对于其灭菌效果需要进行验证。
Objective To verify the sterilizing effects of pre-vacuum, high-temperature and high-pressure steam on orthopedic surgical bags and gynecological surgical bags. Methods Escherichia coli, Bacillus subtilis black mutant spores, Staphylococcus aureus and Bacillus cereus were used as indicator bacteria in a surgical bag for pre-vacuum high-temperature high-pressure steam sterilization in order to verify the pre-vacuum high temperature and pressure Sterilization with steam sterilization. Results The results of pre-vacuum high-temperature autoclave sterilization showed that the sterilization rate of the four test strains in the gynecological surgical package was 100.0%, and the sterilization rate of Escherichia coli, Bacillus subtilis black mutant spores and Staphylococcus aureus in the orthopedic surgery package Both were 100.0%. The sterilization rate of Bacillus cereus in orthopedic surgery package was 85.7%. Conclusion Pre-vacuum high-temperature high-pressure steam sterilization can meet the sterilization requirements of most surgical kits, but its sterilization effect needs to be verified in the practical application.