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为探明Sox基因在物种进化中的保守性及其性别分型,以大鲵雌、雄个体为材料,参照Sox基因HMG-box保守区的序列,设计简并引物扩增,两因子均在雌、雄个体内得到217 bp PCR扩增产物,不存在性别差异。将二者编码的氨基酸序列与NCBI中其他物种Sox基因编码的氨基酸序列进行比对,其中一个基因与人、斑马鱼、家鼠、鸡、热带爪蟾、扬子鳄Sox11基因的同源性均为90%,另一个基因与罗非鱼、鸭嘴兽、家鼠、红鳍东方鲀、斑马鱼、鸡、人、非洲爪蟾和大鲵Sox14基因的同源性均为90%,故根据大鲵的学名,将这2个基因分别命名为adSox11和adSox14c。adSox11和adSox14c在大鲵精巢、卵巢、肾脏和心脏中均有不同程度表达,而在胃和肝脏中几乎不表达。
In order to find out the conservatism and genotyping of Sox gene in the evolution of species, the female and male individuals of Daphnia magna were used as materials to design the degenerate primers according to the sequence of the conserved region of HMG-box in Sox gene. There was no gender difference in the 217 bp PCR products in male. The amino acid sequences of the two genes were compared with the amino acid sequences encoded by Sox gene of other species in NCBI. The homology of one gene with Sox11 gene of human, zebrafish, house mouse, chicken, tropical Xenopus, Chinese alligator was 90 %. The other gene shares 90% homology with the Sox14 gene in tilapia, platypus, house mouse, red-tailed emus, zebrafish, chicken, human, Xenopus and Daunea, These two genes were named adSox11 and adSox14c respectively. AdSox11 and adSox14c were expressed to varying degrees in the testis, ovary, kidney and heart of the big cathartes, but not in the stomach and liver.