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兔附睾液中分离纯化了两个特异性蛋白ＥＳＰ－１和２，分子量分别为４２ＫＤ和２０ＫＤ。应用基因克隆技术，又从人睾丸λ－ｇｔｌｌ表达性。ＤＮＡ基因库中筛选分离出一个１．９Ｋｂ的编码ＥＳＰ－２ｃＤＮＡ片段，为了进一步深入研究ＥＳＰ－２做为抗生育疫苗的可能性，本研究用半固体融合细胞培养基法成功地制备了抗ＥＳＰ－２的单克隆抗体，经三轮筛选后获得阳性克隆３８个，经定量分析ＯＤ值均为０．５以上，其中三株命名为ＥＳＰ－２ｍＡｌ、ＥＳＰ－２ｍＡ３和ＥＳＰ－２ｍＡ９，接种于ＢＡＬＢ／Ｃ小鼠腹腔之后，腹水滴度均达１∶１００，０００。本研究采用体内外结合的被动免疫抗生育实验，即用抗体处理精子后进行体内抗生育实验，用小鼠双侧子宫互为对照和实验，证明ＥＳＰ－２ｍＡ３，ＥＳＰ－２ｍＡ９单抗对小鼠受精有明显的抑制作用，Ｐ＜０．０５和＜０．０１。对上述两株ＥＳＰ－２抗体的抗受精机理需进一步探讨。
Two specific proteins, ESP-1 and 2, were isolated and purified from epididymal fluid of rabbits and their molecular weights were 42KD and 20KD, respectively. Application of gene cloning technology, again from the human testis λ-gtll expression. In order to further study the possibility of ESP-2 as an anti-fertility vaccine, a 1.9Kb fragment of ESP-2cDNA was isolated from the DNA gene library. In this study, we successfully prepared anti-ESP -2 monoclonal antibodies. After three rounds of screening, 38 positive clones were obtained. OD values were all above 0.5 by quantitative analysis. Three of them were named ESP-2mAl, ESP-2mA3 and ESP-2mA9 and inoculated into BALB / C mice after abdominal cavity, the ascites titer reached 1: 100,000. In this study, in vitro and in vivo combination of passive immunization anti-fertility experiment, that is, anti-fertility experiments with anti-sperm in vivo anti-fertility experiment, bilateral mouse uterine mutual control and experiments, ESP-2mA3, ESP- Fertilization was significantly inhibited, P <0.05 and <0.01. Anti-fertilization mechanism of the above two ESP-2 antibodies needs to be further explored.