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用含利福平(400微克/毫升)的马铃薯琼脂培养基(R-PDA)分离5种病原真菌,检出率可达63.8％,无细菌污染,非目标病原的真菌污染率减少34％。对照(PDA)的病原检出率是25％,细菌污染率为89.4％。使用利福平选择分离技术还可简化分离程序,单消毒(70％酒精)较二次消毒(酒精—升汞)的病原检出率高77.2％。同时,利用利福平作为选择分离中的杂菌抑制剂,不影响病菌的生物学性状和致病力。烟草及其它作物的真菌性病害,尤其是根茎病害的病原分离常受细菌污染。而且由于许多细菌存在于植物组织内,表面消毒并不能把它们杀死;而消毒剂内渗又往往会杀死分离的目标病原物。因此,如何防止细菌的污染并获得高的病原检出率,对真菌性病害样本的分离是一个限制性问题。本文报道一项可以解决这一问题的选择分离技术。
Five pathogenic fungi were isolated from potato agar medium (R-PDA) containing rifampicin (400 μg / ml), with a detection rate of 63.8%. No bacterial contamination was found and the fungal contamination rate of non-target pathogen was reduced by 34%. The control (PDA) pathogen detection rate was 25% and the bacterial contamination rate was 89.4%. The use of rifampin selective separation technique also simplifies the separation procedure, with a 77.2% higher detection rate of the pathogen than the single disinfection (70% alcohol) compared to the secondary disinfection (alcohol-mercuric chloride). At the same time, the use of rifampicin as a selective antibacterial inhibitor in isolation does not affect the biological characteristics of pathogens and pathogenicity. Fungal diseases of tobacco and other crops, especially pathogens of rhizome diseases, are often contaminated with bacteria. And because many bacteria are present in plant tissues, surface disinfection does not kill them, and disinfectant infiltration tends to kill isolated target pathogens. Therefore, how to prevent bacterial contamination and to obtain a high detection rate of the pathogen, the separation of fungal disease samples is a limiting issue. This article reports a selective separation technique that can solve this problem.