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以鉴定明确带有褪绿叶斑病毒的新红星试管苗为试材。在培养基中分别加入南板兰根和三氮唑核苷,接入大(5mm)、小(0.5mm)两种茎尖。分化后,经一次继代,进行ELISA检测。结果南板兰根对茎尖分化略有抑制,但其浓度高低没有影响;三氮唑核苷对分化有抑制作用,浓度越高越强,因此应在低浓度下使用。两种药剂均对大茎尖的分化没有影响。经ELISA检测,南板兰根只在高浓度(4支/L)、较小茎尖时(0.5mm).对褪绿叶斑病毒有脱除作用。而三氮唑核苷在本试验中不论浓度高低(10mg/L、20mg/L)和茎尖大小(0.5mm,5mm),对褪绿叶斑病毒均有很好的脱除作用。
In order to identify a clear green leaf spot virus with chlorotic leaf spot test tube material. In the medium were added southern Banlangen and ribavirin, access to large (5mm), small (0.5mm) two kinds of shoot tips. After differentiation, after a succession, ELISA test was performed. Results Southern Banana root tip differentiation slightly inhibited, but its concentration has no effect; ribavirin inhibition of differentiation, the higher the concentration the stronger, it should be used at low concentrations. Both agents have no effect on the differentiation of large shoots. Southern blotting showed that the southern Banlangen was only at high concentration (4 / L) and smaller apical (0.5mm). Chlorotic leaf spot virus removal effect. The ribavirin in this experiment regardless of the concentration of high and low (10mg / L., 20mg / L) and tip size (0.5mm, 5mm), chlorotic leaf spot virus has a good removal.