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目的:观察不同形态硒(L-硒甲基硒代半胱氨酸、亚硒酸钠、富硒酵母)、维生素E、紫胡萝卜提取物(主要成分为花青素)单独和联合用药对H2O2诱导H9c2细胞损伤的保护作用,并探讨其作用机制。方法:将H9c2细胞随机分为11组,即1空白对照组,2H2O2模型组,3L-硒甲基硒代半胱氨酸组(Se MSC),4亚硒酸钠组(SS),5富硒酵母组(SEY),6维生素E组(VE),7紫胡萝卜提取物组(Ant),8VE+Ant联用组,9Se MSC+VE+Ant联用组,10SS+VE+Ant联用组,11SEY+VE+Ant联用组。经不同样品组干预处理后,通过H2O2诱导细胞氧化损伤,检测各组H9c2细胞的存活率,脂质氧化终产物丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力。结果:与模型组相比,不同形态硒、维生素E、紫胡萝卜提取物单独和联合用药组H9c2细胞的存活率明显增加。同时,细胞内MDA的含量降低,SOD、CAT、GSH-Px的抗氧化活力增强,联合用药组的效果优于单独用药组。结论:不同形态硒(L-硒甲基硒代半胱氨酸、亚硒酸钠、富硒酵母)、维生素E、紫胡萝卜提取物均可保护由H2O2诱导引起的H9c2细胞氧化损伤,提高心肌细胞抗氧化能力,联合用药组的效果优于单独用药组,具有明显的抗氧化协同作用。
OBJECTIVE: To observe the effects of different forms of selenium (L-selenomethionine, cysteine, sodium selenite, selenium-enriched yeast), vitamin E and purple carrot extract (the main component is anthocyanin) Induced H9c2 cell injury and explored the mechanism of action. Methods: H9c2 cells were randomly divided into 11 groups: 1 control group, 2H2O2 model group, 3L-selenomethionine group (Se MSC), 4 sodium selenite group (SS) Selenium yeast group (SEY), 6 vitamin E group (VE), 7 purple carrot extract group (Ant), 8VE + Ant combined group, 9Se MSC + VE + Ant combined group, 10SS + VE + Ant combined group , 11SEY + VE + Ant combined group. After intervention by different sample groups, the cell oxidative injury was induced by H2O2, the survival rate of H9c2 cells, the content of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) activity. Results: Compared with the model group, the survival rates of H9c2 cells with different forms of selenium, vitamin E and purple carrot extract alone and in combination group were significantly increased. At the same time, the content of MDA in the cells decreased, and the antioxidant activity of SOD, CAT and GSH-Px increased. The effect of combination group was better than that of group alone. CONCLUSION: Different forms of selenium (L-selenomethionine, cysteine, sodium selenite, selenium-enriched yeast), vitamin E and purple carrot extract can protect H9c2 cells from oxidative damage induced by H2O2, Anti-oxidative capacity of cells in combination group was better than that of single drug group, which had obvious anti-oxidation synergism.