论文部分内容阅读
对慢性乙型肝炎(慢乙肝)来说,了解乙肝病毒(HBV)的增殖部位,在病理学上有重要意义。作者为了解HBV的增殖部位与HBV增殖程度之间的关系,通过原位杂交法检出HBV-DNA,探讨了HBV-DNA阳性细胞在肝组织内的分布。方法和对象:慢乙肝29例,肝穿刺获38份肝组织标本。用原位杂交法、自体射线显影法检测HBV-DNA;其中8例用Southernblot法探讨肝组织内HBV-DNA存在状态。血清中HBeAg用RIA法、DNAP 用Kaplan改良法检测,DNA-P低于200cpm作为阴性。结果:根据原位杂交法,观察肝细胞内以黑点形式存在的HBV-DNA,就HBV-DNA阳性肝细胞的组织分布作了研究,可能有以下四型:Ⅰ、弥散型。切片内HBV-DNA阳性肝细
For chronic hepatitis B (chronic hepatitis B), understanding of the proliferative site of hepatitis B virus (HBV) is pathologically important. To understand the relationship between the proliferative parts of HBV and the degree of HBV proliferation, HBV-DNA was detected by in situ hybridization and the distribution of HBV-DNA positive cells in the liver tissue was investigated. Methods and objects: 29 cases of chronic hepatitis B, liver biopsy specimens of 38 liver tissues. HBV-DNA was detected by in situ hybridization and autoradiography. Eight of them were used to investigate the presence of HBV-DNA in liver tissue by Southern blot. Serum HBeAg with RIA method, DNAP with Kaplan modified detection of DNA-P less than 200cpm as negative. Results: According to the in situ hybridization method, we observed the distribution of HBV-DNA positive hepatocytes by HBV-DNA in hepatocytes. The following four types were possible: Ⅰ, diffuse type. Liver slices of HBV-DNA-positive