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目的研究组蛋白去乙酰化酶4(histone deacetylase 4,HDAC4)和血管内皮生长因子受体-1(vascular endo-thelial growth factor receptor-1,VEGFR-1)在肝癌细胞株HepG2中的表达,观察组蛋白去乙酰化酶抑制剂(histonedeacetylase inhibitors,HDACI)苯丁酸钠(sodium phenylbutyrate,SPB)作用于肝癌细胞株HepG2后,HDAC4、VEG-FR-1之间的变化及对肝癌细胞株HepG2侵袭、黏附作用的影响。方法体外培养肝癌细胞株HepG2,分为对照组、实验组,对照组常规培养,实验组加入苯丁酸钠。分别以免疫细胞化学及Western-blotting检测HDAC4、VEG-FR-1蛋白的表达及蛋白表达变化;应用Transwell小室观察SPB对肝癌细胞株HepG2侵袭力的影响,应用MTT方法测定SPB对HepG2黏附作用的影响。结果体外培养人肝癌细胞株HepG2,免疫细胞化学试验及Western-blot-ting显示HepG2细胞株表达HDAC4和VEGFR-1;Western-blotting显示,实验组SPB抑制HDAC4、VEGFR-1的表达,并呈现时间及剂量依赖关系;应用Transwell小室观察经SPB作用后,实验组、对照组细胞的穿膜细胞数分别为161.80±46.80、329.20±55.99,抑制率达到50.85%,有统计学意义(P<0.01);MTT方法测定黏附作用显示SPB实验组细胞的黏附率较对照组明显下降(P<0.01)。结论 HDAC4、VEGFR-1在肝癌细胞株HepG2中表达,SPB对HDAC4、VEGFR-1的表达有抑制作用;并对HepG2细胞株有抑制其侵袭及黏附的作用。
Objective To investigate the expression of histone deacetylase 4 (HDAC4) and vascular endothelial growth factor receptor-1 (VEGFR-1) in hepatocellular carcinoma cell line HepG2 The changes of HDAC4, VEG-FR-1 and the invasion of hepatoma cell line HepG2 after treated with histonedeacetylase inhibitors (HDACI) sodium phenylbutyrate (SPB) on HepG2 hepatocellular carcinoma cell line , The effect of adhesion. Methods HepG2 cells were cultured in vitro and divided into control group, experimental group and control group. The experimental group was given sodium phenylbutyrate. The expression of HDAC4 and VEG-FR-1 protein and protein expression were detected by immunocytochemistry and Western-blotting respectively. The effect of SPB on the invasiveness of HepG2 hepatoma cell line was observed by Transwell chamber. The adhesion of HepG2 to SPB was determined by MTT assay influences. Results The human hepatocellular carcinoma cell line HepG2 was cultured in vitro. The expression of HDAC4 and VEGFR-1 in HepG2 cells was detected by immunocytochemistry and Western-blot-ting. Western blotting showed that the expression of HDAC4 and VEGFR- (P <0.01). The number of transmembrane cells in experimental group and control group were 161.80 ± 46.80, 329.20 ± 55.99 and 50.85%, respectively, with statistical significance (P <0.01) The MTT assay showed that the adhesion rate of SPB group was significantly lower than that of the control group (P <0.01). Conclusions HDAC4 and VEGFR-1 are expressed in HepG2 hepatocellular carcinoma cell line. SPB can inhibit the expression of HDAC4 and VEGFR-1, and inhibit the invasion and adhesion of HepG2 cells.