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目的探讨炻乳汤含药血清对乳腺癌MDA-MB-231细胞增殖及迁移的影响。方法以高、中、低剂量炻乳汤含药血清加入体外培养的三阴性乳腺癌细胞系MDA-MB-231细胞,MTT法检测其对MDA-MB-231细胞增殖的影响,Transwell细胞迁移实验观察其对MDA-MB-231细胞迁移的影响。结果低剂量组、中剂量组与空白对照组对比,增值率下降;高剂量组72 h有使肿瘤细胞凋亡的作用(P<0.01);三组均明显抑制细胞迁移(P<0.01)。结论炻乳汤有抑制MDA-MB-231细胞增殖及迁移作用。
Objective To investigate the effects of serum containing Yanshou Decoction on the proliferation and migration of breast cancer MDA-MB-231 cells. Methods High, medium and low doses of milk soup containing serum added to the culture of triple negative breast cancer cell line MDA-MB-231 cells, MTT assay of MDA-MB-231 cells proliferation, Transwell cell migration assay The effect of MDA on the migration of MDA-MB-231 cells was observed. Results Compared with the blank control group, the proliferation rate of the low-dose group and the medium-dose group decreased, while the apoptosis rate of the high-dose group was higher than that of the blank control group (P <0.01). The migration of the three groups significantly inhibited the cell migration (P <0.01). Conclusion Sanyang decoction can inhibit the proliferation and migration of MDA-MB-231 cells.