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目的研究临床分离的耐甲氧西林金黄色葡萄球菌对碘伏消毒剂的抗性变化及其抗消毒剂基因携带情况。方法采用肉汤稀释法和基因检测技术,对临床分离的耐甲氧西林金黄色葡萄球菌抗消毒剂基因携带情况及其对碘伏消毒剂抗性变化进行了观察。结果共检出耐甲氧西林金黄色葡萄球菌152株,其中有11株携带qacA/B基因,携带率为7.24%。碘伏消毒剂对携带qacA/B基因MRSA菌株的MIC值为100~300 mg/L范围,有4株菌的MIC值为300 mg/L。碘伏消毒剂对非携带qacA/B基因的MRSA菌株MIC值为100~200 mg/L范围。结论携带qacA/B基因的MRSA菌株对碘伏消毒剂的抗性有增大趋势,其抗性可能与qacA/B基因有关。
Objective To study the changes of resistance to methicillin-resistant Staphylococcus aureus to iodophor disinfectant and the gene delivery of anti-disinfectants in clinical isolates. Methods The antibiotic resistance of methicillin-resistant Staphylococcus aureus isolates was detected by using the method of broth dilution and gene detection. Results A total of 152 strains of methicillin - resistant Staphylococcus aureus were detected. Among them, 11 strains carried qacA / B gene with a carrying rate of 7.24%. The MIC value of iodophor disinfectant to MRSA strains carrying qacA / B gene was in the range of 100-300 mg / L, and the MIC value of four strains was 300 mg / L. The MIC value of iodophor disinfectant against MRSA strains carrying qacA / B gene was in the range of 100-200 mg / L. Conclusion The resistance of MRSA strains carrying qacA / B to iodophor disinfectant tends to increase, and its resistance may be related to the qacA / B gene.