论文部分内容阅读
本文运用连续录像手段和荧光测钙技术研究丁胺卡那霉素对离体耳蜗外毛细胞运动及胞内游离钙浓度的影响。结果显示：（１）正常状态下外毛细胞内游离钙的荧光比值为１．３９±０．０９（ｘ±ｓｘ，ｎ＝１５）；从等渗环境进入低渗环境外毛细胞变短变粗（Ｐ＜０．０５，ｎ＝７。（２）丁胺卡那霉素（６．６７ｍｇ／ｍｌ）可使外毛细胞胞膜皱缩内陷，局部直径明显变细（Ｐ＜０．０１，ｎ＝１９），即使在低渗环境中也难以恢复原状。（３）丁胺卡那霉素（３．２３ｍｇ／ｍｌ）可瞬间降低细胞内钙离子浓度，使荧光比值降低１４．６８±２．０５％（Ｐ＜０．０１，ｎ＝１２）。提示丁胺卡那霉素的耳毒性作用机制可能通过降低细胞内游离钙浓度，进而影响外毛细胞钙依赖性的“慢能动性”运动
In this paper, using continuous video recording and fluorescence detection of calcium technology in vitro amikacin on cochlear outer hair cell motility and intracellular free calcium concentration. The results showed that: (1) The fluorescence ratio of free calcium in the outer hair cells under normal conditions was 1.39 ± 0.09 (x ± sx, n = 15) Crude (P <0.05, n = 7. (2) Amikacin (6.67mg / ml) can make the outer hair cell membrane shrinkage invaginated, the local diameter was significantly thinner (P <0. 01, n = 19), even in the hypotonic environment is difficult to restore the status quo. (3) amikacin (3.23mg / ml) can instantly reduce intracellular calcium concentration, the fluorescence ratio decreased 14.68 ± 2.05% (P <0.01, n = 12), suggesting that the mechanism of ototoxicity of amikacin may affect the calcium-dependent slow motility of outer hair cells by decreasing intracellular free calcium concentration "movement