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目的研究人类白细胞抗原G(HLA-G)非编码区5′URR基因单核苷酸多态性和3′UTR 14 bp基因插入/缺失单核苷酸多态性与不明原因反复性流产相关性。方法病例组为不明原因反复性流产史患者(流产次数≥2)112例和有正常妊娠史无流产史的妇女(对照组)112例,留取EDTA-K2抗凝全血提取DNA。采用PCR技术扩增HLA-G 3′UTR 14 bp和HLA-G5′URR基因,3′UTR 14 bp扩增产物通过凝胶电泳分型,直接计数法比较2组间基因型频率、等位基因频率分布。HLA-G 5′URR基因PCR产物送公司测序,在线SHEsis软件比较2组间基因型频率、等位基因频率分布。结果 HLA-G 3′UTR 14 bp插入/缺失多态性基因型频率和等位基因频率在2组间差异无统计学意义(P>0.05),HLA-G 5′URR等位基因-725G在2组间差异有统计学意义(P<0.05)。HLA-G 5′URR-725GG基因型频率在2组间差异有统计学意义(P<0.05)。结论 HLA-G 5′URR-725G等位基因和HLA-G 5′URR-725GG基因型可能是不明原因反复流产易感性基因,而HLA-G 5′URR-725C等位基因可能是不明原因反复流产保护性基因。
Objective To investigate the association between single nucleotide polymorphisms in 5’UTR of human leukocyte antigen G (HLA-G) non-coding region and single nucleotide polymorphisms in 3’UTR 14 bp gene and unexplained repeated abortion . Methods One hundred and twelve patients with recurrent miscarriage history (miscarriage≥2) and 112 women with normal pregnancy history without miscarriage (control group) were enrolled in this study. EDTA-K2 anticoagulant whole blood was collected to extract DNA. The 14 bp of HLA-G 3’UTR and HLA-G5’URR gene were amplified by PCR and the 14 bp of 3’UTR was amplified by gel electrophoresis. The genotype frequency and allele Frequency distribution. The HLA-G 5’UTR gene PCR products were sent to the company for sequencing. The online SHEsis software was used to compare genotype frequency and allele frequency distribution between the two groups. Results There was no significant difference in frequencies of genotypes and alleles of HLA-G 3’UTR 14 bp insert / deletion polymorphisms between the two groups (P> 0.05). HLA-G 5’URR allele -725G was The difference between the two groups was statistically significant (P <0.05). HLA-G 5’UTR-725GG genotype frequencies between the two groups were statistically significant (P <0.05). Conclusion HLA-G 5’URR-725G allele and HLA-G 5’URR-725GG genotype may be unexplained recurrent miscarriage susceptibility genes, while HLA-G 5’URR-725C allele may be unknown repeated Abortion protective genes.